Structure. 2014 Apr 8;22(4):512-4. doi: 10.1016/j.str.2014.03.005.

Tracking allosteric propagation with HX-MS

The allosterically-induced outcome of nitric oxide (NO) binding to soluble guanylate cyclase (sGC) is well known but poorly understood. As described in this issue of Structure, Underbakke and coworkers apply a powerful hydrogen/deuterium exchange method to follow the key structural elements of the pathway between NO binding and sGC catalytic site activation.